Construct: shRNA TRCN0000080495

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021436.1-1265s1c1
DNA Barcode:
GCAGTACAGATCGCCATTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmeff1 (230157)
Gene Description:
transmembrane protein with EGF-like and two follistatin-like domains 1
Transcript:
RefSeq NM_021436.1 (NON-CURRENT)
Match location:
Position 1265 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080495)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTACAGATCGCCATTATA
Hairpin Sequence:
5'-CCGG-GCAGTACAGATCGCCATTATA-CTCGAG-TATAATGGCGATCTGTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTACAGATCGCCATTATACTCGAGTATAATGGCGATCTGTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTACAGATCGCCATTATACTCGAGTATAATGGCGATCTGTACTGC-3'

Other clones with same target sequence:

(none)