Construct: shRNA TRCN0000080504

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175628.2-3091s1c1
DNA Barcode:
CGGGTTACCAAAGACAATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
A2m (232345)
Gene Description:
alpha-2-macroglobulin
Transcript:
RefSeq NM_175628.2 (NON-CURRENT)
Match location:
Position 3091 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080504)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGTTACCAAAGACAATTAA
Hairpin Sequence:
5'-CCGG-CGGGTTACCAAAGACAATTAA-CTCGAG-TTAATTGTCTTTGGTAACCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGTTACCAAAGACAATTAACTCGAGTTAATTGTCTTTGGTAACCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGTTACCAAAGACAATTAACTCGAGTTAATTGTCTTTGGTAACCCG-3'

Other clones with same target sequence:

(none)