Construct: shRNA TRCN0000080560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_149387.5-1567s1c1
DNA Barcode:
CCATCCAGTGAGGGAAGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdc14a (229776)
Gene Description:
CDC14 cell division cycle 14A
Transcript:
RefSeq XM_149387.5 (NON-CURRENT)
Match location:
Position 1567 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCCAGTGAGGGAAGTATT
Hairpin Sequence:
5'-CCGG-CCATCCAGTGAGGGAAGTATT-CTCGAG-AATACTTCCCTCACTGGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCCAGTGAGGGAAGTATTCTCGAGAATACTTCCCTCACTGGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCCAGTGAGGGAAGTATTCTCGAGAATACTTCCCTCACTGGATGG-3'

Other clones with same target sequence:

(none)