Construct: shRNA TRCN0000080574

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033264.1-914s1c1
DNA Barcode:
CGGTTTATCTTGAAGCGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arpp21 (74100)
Gene Description:
cyclic AMP-regulated phosphoprotein, 21
Transcript:
RefSeq NM_033264.1 (NON-CURRENT)
Match location:
Position 914 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080574)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTTATCTTGAAGCGAGAT
Hairpin Sequence:
5'-CCGG-CGGTTTATCTTGAAGCGAGAT-CTCGAG-ATCTCGCTTCAAGATAAACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTTATCTTGAAGCGAGATCTCGAGATCTCGCTTCAAGATAAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTTATCTTGAAGCGAGATCTCGAGATCTCGCTTCAAGATAAACCG-3'

Other clones with same target sequence:

(none)