Construct: shRNA TRCN0000080613

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011523.1-3935s1c1
DNA Barcode:
CTGTAGAGTACGTTGGTACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Synj2 (20975)
Gene Description:
synaptojanin 2
Transcript:
RefSeq NM_011523.1 (NON-CURRENT)
Match location:
Position 3935 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080613)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTAGAGTACGTTGGTACAA
Hairpin Sequence:
5'-CCGG-CTGTAGAGTACGTTGGTACAA-CTCGAG-TTGTACCAACGTACTCTACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTAGAGTACGTTGGTACAACTCGAGTTGTACCAACGTACTCTACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTAGAGTACGTTGGTACAACTCGAGTTGTACCAACGTACTCTACAG-3'

Other clones with same target sequence:

  1. TRCN0000288185