Construct: shRNA TRCN0000080624

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019837.1-387s1c1
DNA Barcode:
CGCCATCCAGACCGATGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nudt3 (56409)
Gene Description:
nudix (nucleotide diphosphate linked moiety X)-type motif 3
Transcript:
RefSeq NM_019837.1 (NON-CURRENT)
Match location:
Position 387 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080624)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCATCCAGACCGATGGATT
Hairpin Sequence:
5'-CCGG-CGCCATCCAGACCGATGGATT-CTCGAG-AATCCATCGGTCTGGATGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCATCCAGACCGATGGATTCTCGAGAATCCATCGGTCTGGATGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCATCCAGACCGATGGATTCTCGAGAATCCATCGGTCTGGATGGCG-3'

Other clones with same target sequence:

  1. TRCN0000334578