Construct: shRNA TRCN0000080643

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_134427.4-3094s1c1
DNA Barcode:
CCAGTCTATCTGTTCTCCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Inpp4b (234515)
Gene Description:
inositol polyphosphate-4-phosphatase, type II
Transcript:
RefSeq XM_134427.4 (NON-CURRENT)
Match location:
Position 3094 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080643)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCTATCTGTTCTCCTTT
Hairpin Sequence:
5'-CCGG-CCAGTCTATCTGTTCTCCTTT-CTCGAG-AAAGGAGAACAGATAGACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCTATCTGTTCTCCTTTCTCGAGAAAGGAGAACAGATAGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCTATCTGTTCTCCTTTCTCGAGAAAGGAGAACAGATAGACTGG-3'

Other clones with same target sequence:

(none)