Construct: shRNA TRCN0000080764

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_488032.1-657s1c1
DNA Barcode:
CCATGATATTGAAAGGTCTGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC435974 (435974)
Gene Description:
similar to protein phosphatase 2C alpha 3
Transcript:
RefSeq XM_488032.1 (NON-CURRENT)
Match location:
Position 657 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080764)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGATATTGAAAGGTCTGA
Hairpin Sequence:
5'-CCGG-CCATGATATTGAAAGGTCTGA-CTCGAG-TCAGACCTTTCAATATCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGATATTGAAAGGTCTGACTCGAGTCAGACCTTTCAATATCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGATATTGAAAGGTCTGACTCGAGTCAGACCTTTCAATATCATGG-3'

Other clones with same target sequence:

(none)