Construct: shRNA TRCN0000080816

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011851.2-843s1c1
DNA Barcode:
CCATTCATAGTCACCGCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nt5e (23959)
Gene Description:
5' nucleotidase, ecto
Transcript:
RefSeq NM_011851.2 (NON-CURRENT)
Match location:
Position 843 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080816)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTCATAGTCACCGCAGAT
Hairpin Sequence:
5'-CCGG-CCATTCATAGTCACCGCAGAT-CTCGAG-ATCTGCGGTGACTATGAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTCATAGTCACCGCAGATCTCGAGATCTGCGGTGACTATGAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTCATAGTCACCGCAGATCTCGAGATCTGCGGTGACTATGAATGG-3'

Other clones with same target sequence:

(none)