Construct: shRNA TRCN0000080838

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_284491.4-1806s1c1
DNA Barcode:
CCCTAACTACTTACACAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp2r1b (73699)
Gene Description:
protein phosphatase 2, regulatory subunit A, beta
Transcript:
RefSeq XM_284491.4 (NON-CURRENT)
Match location:
Position 1806 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080838)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAACTACTTACACAGAAT
Hairpin Sequence:
5'-CCGG-CCCTAACTACTTACACAGAAT-CTCGAG-ATTCTGTGTAAGTAGTTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAACTACTTACACAGAATCTCGAGATTCTGTGTAAGTAGTTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAACTACTTACACAGAATCTCGAGATTCTGTGTAAGTAGTTAGGG-3'

Other clones with same target sequence:

(none)