Construct: shRNA TRCN0000080848

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146067.2-2187s1c1
DNA Barcode:
GCCCATTATTAGGAAGCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpped1 (223978)
Gene Description:
calcineurin-like phosphoesterase domain containing 1
Transcript:
RefSeq NM_146067.2 (NON-CURRENT)
Match location:
Position 2187 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080848)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATTATTAGGAAGCACAA
Hairpin Sequence:
5'-CCGG-GCCCATTATTAGGAAGCACAA-CTCGAG-TTGTGCTTCCTAATAATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATTATTAGGAAGCACAACTCGAGTTGTGCTTCCTAATAATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATTATTAGGAAGCACAACTCGAGTTGTGCTTCCTAATAATGGGC-3'

Other clones with same target sequence:

  1. TRCN0000325105