Construct: shRNA TRCN0000080882

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008247.1-1167s1c1
DNA Barcode:
CTTCAAGGACACACATTCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plpp1 (19012)
Gene Description:
phospholipid phosphatase 1
Transcript:
RefSeq NM_008247.1 (NON-CURRENT)
Match location:
Position 1167 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080882)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAAGGACACACATTCTTA
Hairpin Sequence:
5'-CCGG-CTTCAAGGACACACATTCTTA-CTCGAG-TAAGAATGTGTGTCCTTGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAAGGACACACATTCTTACTCGAGTAAGAATGTGTGTCCTTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAAGGACACACATTCTTACTCGAGTAAGAATGTGTGTCCTTGAAG-3'

Other clones with same target sequence:

(none)