Construct: shRNA TRCN0000080894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177242.3-898s1c1
DNA Barcode:
CGCTGCTGATAGCACATCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pptc7 (320717)
Gene Description:
PTC7 protein phosphatase homolog
Transcript:
RefSeq NM_177242.3 (NON-CURRENT)
Match location:
Position 898 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGCTGATAGCACATCTTT
Hairpin Sequence:
5'-CCGG-CGCTGCTGATAGCACATCTTT-CTCGAG-AAAGATGTGCTATCAGCAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGCTGATAGCACATCTTTCTCGAGAAAGATGTGCTATCAGCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGCTGATAGCACATCTTTCTCGAGAAAGATGTGCTATCAGCAGCG-3'

Other clones with same target sequence:

  1. TRCN0000318132