Construct: shRNA TRCN0000080911

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_136364.6-2877s1c1
DNA Barcode:
GCATGGGTAACGAGATGGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dusp27 (240892)
Gene Description:
dual specificity phosphatase 27 (putative)
Transcript:
RefSeq XM_136364.6 (NON-CURRENT)
Match location:
Position 2877 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080911)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGGGTAACGAGATGGATA
Hairpin Sequence:
5'-CCGG-GCATGGGTAACGAGATGGATA-CTCGAG-TATCCATCTCGTTACCCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGGGTAACGAGATGGATACTCGAGTATCCATCTCGTTACCCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGGGTAACGAGATGGATACTCGAGTATCCATCTCGTTACCCATGC-3'

Other clones with same target sequence:

(none)