Construct: shRNA TRCN0000080918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181750.2-3883s1c1
DNA Barcode:
CCCATCTGATTGGCTTGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
R3hdm1 (226412)
Gene Description:
R3H domain containing 1
Transcript:
RefSeq NM_181750.2 (CURRENT)
Match location:
Position 3883 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCTGATTGGCTTGTTAA
Hairpin Sequence:
5'-CCGG-CCCATCTGATTGGCTTGTTAA-CTCGAG-TTAACAAGCCAATCAGATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCTGATTGGCTTGTTAACTCGAGTTAACAAGCCAATCAGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCTGATTGGCTTGTTAACTCGAGTTAACAAGCCAATCAGATGGG-3'

Other clones with same target sequence:

  1. TRCN0000324816