Construct: shRNA TRCN0000080954

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177215.3-2523s1c1
DNA Barcode:
CCTGAGACAATTCCTGGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ocrl (320634)
Gene Description:
OCRL, inositol polyphosphate-5-phosphatase
Transcript:
RefSeq NM_177215.3 (CURRENT)
Match location:
Position 2523 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080954)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGACAATTCCTGGCAAT
Hairpin Sequence:
5'-CCGG-CCTGAGACAATTCCTGGCAAT-CTCGAG-ATTGCCAGGAATTGTCTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGACAATTCCTGGCAATCTCGAGATTGCCAGGAATTGTCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGACAATTCCTGGCAATCTCGAGATTGCCAGGAATTGTCTCAGG-3'

Other clones with same target sequence:

(none)