Construct: shRNA TRCN0000080976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026295.2-2023s1c1
DNA Barcode:
GCACCCAACAAACTTTCCTGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctdp1 (67655)
Gene Description:
CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1
Transcript:
RefSeq NM_026295.2 (CURRENT)
Match location:
Position 2023 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCCAACAAACTTTCCTGT
Hairpin Sequence:
5'-CCGG-GCACCCAACAAACTTTCCTGT-CTCGAG-ACAGGAAAGTTTGTTGGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCCAACAAACTTTCCTGTCTCGAGACAGGAAAGTTTGTTGGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCCAACAAACTTTCCTGTCTCGAGACAGGAAAGTTTGTTGGGTGC-3'

Other clones with same target sequence:

(none)