Construct: shRNA TRCN0000080993

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485489.1-346s1c1
DNA Barcode:
CCCAGCTCAAAGATGCCACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC433793 (433793)
Gene Description:
similar to Protein phosphatase 2, regulatory subunit B (B56), alpha isoform
Transcript:
RefSeq XM_485489.1 (NON-CURRENT)
Match location:
Position 346 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000080993)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGCTCAAAGATGCCACTT
Hairpin Sequence:
5'-CCGG-CCCAGCTCAAAGATGCCACTT-CTCGAG-AAGTGGCATCTTTGAGCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGCTCAAAGATGCCACTTCTCGAGAAGTGGCATCTTTGAGCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGCTCAAAGATGCCACTTCTCGAGAAGTGGCATCTTTGAGCTGGG-3'

Other clones with same target sequence:

(none)