Construct: shRNA TRCN0000081017

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484899.2-945s1c1
DNA Barcode:
GAGTTGTATAAGCACTTGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC433336 (433336)
Gene Description:
similar to protein phosphatase 1B, magnesium dependent, beta isoform
Transcript:
RefSeq XM_484899.2 (NON-CURRENT)
Match location:
Position 945 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081017)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTGTATAAGCACTTGGAA
Hairpin Sequence:
5'-CCGG-GAGTTGTATAAGCACTTGGAA-CTCGAG-TTCCAAGTGCTTATACAACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTGTATAAGCACTTGGAACTCGAGTTCCAAGTGCTTATACAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTGTATAAGCACTTGGAACTCGAGTTCCAAGTGCTTATACAACTC-3'

Other clones with same target sequence:

(none)