Construct: shRNA TRCN0000081058

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008913.1-1751s1c1
DNA Barcode:
GCCAGGAATTGGATTCAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp3ca (19055)
Gene Description:
protein phosphatase 3, catalytic subunit, alpha isoform
Transcript:
RefSeq NM_008913.1 (NON-CURRENT)
Match location:
Position 1751 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081058)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGAATTGGATTCAGTTT
Hairpin Sequence:
5'-CCGG-GCCAGGAATTGGATTCAGTTT-CTCGAG-AAACTGAATCCAATTCCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGAATTGGATTCAGTTTCTCGAGAAACTGAATCCAATTCCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGAATTGGATTCAGTTTCTCGAGAAACTGAATCCAATTCCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000309716