Construct: shRNA TRCN0000081095

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_131156.5-737s1c1
DNA Barcode:
CTGCAATTAGCCACAAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plppr5 (75769)
Gene Description:
phospholipid phosphatase related 5
Transcript:
RefSeq XM_131156.5 (NON-CURRENT)
Match location:
Position 737 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081095)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCAATTAGCCACAAGAGAT
Hairpin Sequence:
5'-CCGG-CTGCAATTAGCCACAAGAGAT-CTCGAG-ATCTCTTGTGGCTAATTGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCAATTAGCCACAAGAGATCTCGAGATCTCTTGTGGCTAATTGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCAATTAGCCACAAGAGATCTCGAGATCTCTTGTGGCTAATTGCAG-3'

Other clones with same target sequence:

(none)