Construct: shRNA TRCN0000081162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011364.2-456s1c1
DNA Barcode:
ACAGGGAGAAGAGATTCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh2d1a (20400)
Gene Description:
SH2 domain containing 1A
Transcript:
RefSeq NM_011364.2 (NON-CURRENT)
Match location:
Position 456 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGGGAGAAGAGATTCTGAT
Hairpin Sequence:
5'-CCGG-ACAGGGAGAAGAGATTCTGAT-CTCGAG-ATCAGAATCTCTTCTCCCTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGGGAGAAGAGATTCTGATCTCGAGATCAGAATCTCTTCTCCCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGGGAGAAGAGATTCTGATCTCGAGATCAGAATCTCTTCTCCCTGT-3'

Other clones with same target sequence:

(none)