Construct: shRNA TRCN0000081187

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144880.3-1127s1c1
DNA Barcode:
AGATTCTTGGAGAGTCCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp2r5a (226849)
Gene Description:
protein phosphatase 2, regulatory subunit B', alpha
Transcript:
RefSeq NM_144880.3 (NON-CURRENT)
Match location:
Position 1127 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081187)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATTCTTGGAGAGTCCTGAT
Hairpin Sequence:
5'-CCGG-AGATTCTTGGAGAGTCCTGAT-CTCGAG-ATCAGGACTCTCCAAGAATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATTCTTGGAGAGTCCTGATCTCGAGATCAGGACTCTCCAAGAATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATTCTTGGAGAGTCCTGATCTCGAGATCAGGACTCTCCAAGAATCT-3'

Other clones with same target sequence:

(none)