Construct: shRNA TRCN0000081189

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024438.2-486s1c1
DNA Barcode:
CCTCAGTGAGTTTACATATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dusp19 (68082)
Gene Description:
dual specificity phosphatase 19
Transcript:
RefSeq NM_024438.2 (NON-CURRENT)
Match location:
Position 486 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081189)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGTGAGTTTACATATAA
Hairpin Sequence:
5'-CCGG-CCTCAGTGAGTTTACATATAA-CTCGAG-TTATATGTAAACTCACTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGTGAGTTTACATATAACTCGAGTTATATGTAAACTCACTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGTGAGTTTACATATAACTCGAGTTATATGTAAACTCACTGAGG-3'

Other clones with same target sequence:

  1. TRCN0000335655