Construct: shRNA TRCN0000081195

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013512.1-201s1c1
DNA Barcode:
CCTCGTGGAGATAGATTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Epb41l4a (13824)
Gene Description:
erythrocyte membrane protein band 4.1 like 4a
Transcript:
RefSeq NM_013512.1 (NON-CURRENT)
Match location:
Position 201 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081195)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGTGGAGATAGATTATTT
Hairpin Sequence:
5'-CCGG-CCTCGTGGAGATAGATTATTT-CTCGAG-AAATAATCTATCTCCACGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGTGGAGATAGATTATTTCTCGAGAAATAATCTATCTCCACGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGTGGAGATAGATTATTTCTCGAGAAATAATCTATCTCCACGAGG-3'

Other clones with same target sequence:

(none)