Construct: shRNA TRCN0000081205

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_489610.1-2304s1c1
DNA Barcode:
CCTTCCGAATGAAGAGGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Synj1 (104015)
Gene Description:
synaptojanin 1
Transcript:
RefSeq XM_489610.1 (NON-CURRENT)
Match location:
Position 2304 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081205)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCGAATGAAGAGGTTAA
Hairpin Sequence:
5'-CCGG-CCTTCCGAATGAAGAGGTTAA-CTCGAG-TTAACCTCTTCATTCGGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCGAATGAAGAGGTTAACTCGAGTTAACCTCTTCATTCGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCGAATGAAGAGGTTAACTCGAGTTAACCTCTTCATTCGGAAGG-3'

Other clones with same target sequence:

(none)