Construct: shRNA TRCN0000081225

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027722.2-598s1c1
DNA Barcode:
GCTGGAGTCAAAGGAAAGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nudt4 (71207)
Gene Description:
nudix (nucleoside diphosphate linked moiety X)-type motif 4
Transcript:
RefSeq NM_027722.2 (NON-CURRENT)
Match location:
Position 598 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081225)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAGTCAAAGGAAAGCTA
Hairpin Sequence:
5'-CCGG-GCTGGAGTCAAAGGAAAGCTA-CTCGAG-TAGCTTTCCTTTGACTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAGTCAAAGGAAAGCTACTCGAGTAGCTTTCCTTTGACTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAGTCAAAGGAAAGCTACTCGAGTAGCTTTCCTTTGACTCCAGC-3'

Other clones with same target sequence:

(none)