Construct: shRNA TRCN0000081274

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026421.1-919s1c1
DNA Barcode:
CGACGAGAAGACATACTACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Enoph1 (67870)
Gene Description:
enolase-phosphatase 1
Transcript:
RefSeq NM_026421.1 (NON-CURRENT)
Match location:
Position 919 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081274)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGAGAAGACATACTACAA
Hairpin Sequence:
5'-CCGG-CGACGAGAAGACATACTACAA-CTCGAG-TTGTAGTATGTCTTCTCGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGAGAAGACATACTACAACTCGAGTTGTAGTATGTCTTCTCGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGAGAAGACATACTACAACTCGAGTTGTAGTATGTCTTCTCGTCG-3'

Other clones with same target sequence:

  1. TRCN0000288490