Construct: shRNA TRCN0000081308

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020271.2-1375s1c1
DNA Barcode:
GCTTAAAGAATGCCTTGCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdxp (57028)
Gene Description:
pyridoxal (pyridoxine, vitamin B6) phosphatase
Transcript:
RefSeq NM_020271.2 (NON-CURRENT)
Match location:
Position 1375 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081308)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAAAGAATGCCTTGCATA
Hairpin Sequence:
5'-CCGG-GCTTAAAGAATGCCTTGCATA-CTCGAG-TATGCAAGGCATTCTTTAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAAAGAATGCCTTGCATACTCGAGTATGCAAGGCATTCTTTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAAAGAATGCCTTGCATACTCGAGTATGCAAGGCATTCTTTAAGC-3'

Other clones with same target sequence:

  1. TRCN0000288481