Construct: shRNA TRCN0000081317

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181320.2-934s1c1
DNA Barcode:
CCAACTCGAATTCTTCCCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dusp16 (70686)
Gene Description:
dual specificity phosphatase 16
Transcript:
RefSeq NM_181320.2 (NON-CURRENT)
Match location:
Position 934 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081317)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACTCGAATTCTTCCCAAT
Hairpin Sequence:
5'-CCGG-CCAACTCGAATTCTTCCCAAT-CTCGAG-ATTGGGAAGAATTCGAGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACTCGAATTCTTCCCAATCTCGAGATTGGGAAGAATTCGAGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACTCGAATTCTTCCCAATCTCGAGATTGGGAAGAATTCGAGTTGG-3'

Other clones with same target sequence:

(none)