Construct: shRNA TRCN0000081326

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030692.1-73s1c1
DNA Barcode:
GCTTGTGATGATGGAGCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sacm1l (83493)
Gene Description:
SAC1 suppressor of actin mutations 1-like (yeast)
Transcript:
RefSeq NM_030692.1 (NON-CURRENT)
Match location:
Position 73 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081326)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGTGATGATGGAGCAGAT
Hairpin Sequence:
5'-CCGG-GCTTGTGATGATGGAGCAGAT-CTCGAG-ATCTGCTCCATCATCACAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGTGATGATGGAGCAGATCTCGAGATCTGCTCCATCATCACAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGTGATGATGGAGCAGATCTCGAGATCTGCTCCATCATCACAAGC-3'

Other clones with same target sequence:

  1. TRCN0000332364