Construct: shRNA TRCN0000081329

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_487314.1-61s1c1
DNA Barcode:
GAAGTCCATGATCTTCCTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC435386 (435386)
Gene Description:
similar to Fhit protein
Transcript:
RefSeq XM_487314.1 (NON-CURRENT)
Match location:
Position 61 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081329)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTCCATGATCTTCCTGTT
Hairpin Sequence:
5'-CCGG-GAAGTCCATGATCTTCCTGTT-CTCGAG-AACAGGAAGATCATGGACTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTCCATGATCTTCCTGTTCTCGAGAACAGGAAGATCATGGACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTCCATGATCTTCCTGTTCTCGAGAACAGGAAGATCATGGACTTC-3'

Other clones with same target sequence:

(none)