Construct: shRNA TRCN0000081351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484831.1-408s1c1
DNA Barcode:
CTAGAGCAAACCATCACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
D430013K02 (329095)
Gene Description:
hypothetical protein D430013K02
Transcript:
RefSeq XM_484831.1 (NON-CURRENT)
Match location:
Position 408 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGAGCAAACCATCACTTTA
Hairpin Sequence:
5'-CCGG-CTAGAGCAAACCATCACTTTA-CTCGAG-TAAAGTGATGGTTTGCTCTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGAGCAAACCATCACTTTACTCGAGTAAAGTGATGGTTTGCTCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGAGCAAACCATCACTTTACTCGAGTAAAGTGATGGTTTGCTCTAG-3'

Other clones with same target sequence:

(none)