Construct: shRNA TRCN0000081374

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175523.3-460s1c1
DNA Barcode:
CCTAGCATCAAGTACGGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppm1k (243382)
Gene Description:
protein phosphatase 1K (PP2C domain containing)
Transcript:
RefSeq NM_175523.3 (NON-CURRENT)
Match location:
Position 460 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081374)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGCATCAAGTACGGCAAA
Hairpin Sequence:
5'-CCGG-CCTAGCATCAAGTACGGCAAA-CTCGAG-TTTGCCGTACTTGATGCTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGCATCAAGTACGGCAAACTCGAGTTTGCCGTACTTGATGCTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGCATCAAGTACGGCAAACTCGAGTTTGCCGTACTTGATGCTAGG-3'

Other clones with same target sequence:

(none)