Construct: shRNA TRCN0000081397

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_135153.5-1226s1c1
DNA Barcode:
CCGTTATTAACCATAAACCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp2r3a (235542)
Gene Description:
protein phosphatase 2, regulatory subunit B'', alpha
Transcript:
RefSeq XM_135153.5 (NON-CURRENT)
Match location:
Position 1226 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081397)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTATTAACCATAAACCAT
Hairpin Sequence:
5'-CCGG-CCGTTATTAACCATAAACCAT-CTCGAG-ATGGTTTATGGTTAATAACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTATTAACCATAAACCATCTCGAGATGGTTTATGGTTAATAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTATTAACCATAAACCATCTCGAGATGGTTTATGGTTAATAACGG-3'

Other clones with same target sequence:

(none)