Construct: shRNA TRCN0000081445

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029810.2-1582s1c1
DNA Barcode:
CGCACGTCAGTGGATTTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nt5c2 (76952)
Gene Description:
5'-nucleotidase, cytosolic II
Transcript:
RefSeq NM_029810.2 (NON-CURRENT)
Match location:
Position 1582 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081445)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCACGTCAGTGGATTTCAAA
Hairpin Sequence:
5'-CCGG-CGCACGTCAGTGGATTTCAAA-CTCGAG-TTTGAAATCCACTGACGTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCACGTCAGTGGATTTCAAACTCGAGTTTGAAATCCACTGACGTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCACGTCAGTGGATTTCAAACTCGAGTTTGAAATCCACTGACGTGCG-3'

Other clones with same target sequence:

  1. TRCN0000327333