Construct: shRNA TRCN0000081480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028099.2-421s1c1
DNA Barcode:
CGCTATTATAAAGTAGAGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dusp11 (72102)
Gene Description:
dual specificity phosphatase 11 (RNA/RNP complex 1-interacting)
Transcript:
RefSeq NM_028099.2 (NON-CURRENT)
Match location:
Position 421 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTATTATAAAGTAGAGGAT
Hairpin Sequence:
5'-CCGG-CGCTATTATAAAGTAGAGGAT-CTCGAG-ATCCTCTACTTTATAATAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTATTATAAAGTAGAGGATCTCGAGATCCTCTACTTTATAATAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTATTATAAAGTAGAGGATCTCGAGATCCTCTACTTTATAATAGCG-3'

Other clones with same target sequence:

(none)