Construct: shRNA TRCN0000081510

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021431.1-556s1c1
DNA Barcode:
CACGCCGAGTACCTGGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nudt11 (58242)
Gene Description:
nudix (nucleoside diphosphate linked moiety X)-type motif 11
Transcript:
RefSeq NM_021431.1 (NON-CURRENT)
Match location:
Position 556 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081510)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGCCGAGTACCTGGAGAAA
Hairpin Sequence:
5'-CCGG-CACGCCGAGTACCTGGAGAAA-CTCGAG-TTTCTCCAGGTACTCGGCGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGCCGAGTACCTGGAGAAACTCGAGTTTCTCCAGGTACTCGGCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGCCGAGTACCTGGAGAAACTCGAGTTTCTCCAGGTACTCGGCGTG-3'

Other clones with same target sequence:

(none)