Construct: shRNA TRCN0000081528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172339.1-4153s1c1
DNA Barcode:
GCAGTACACAACCCTCAGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snapc4 (227644)
Gene Description:
small nuclear RNA activating complex, polypeptide 4
Transcript:
RefSeq NM_172339.1 (NON-CURRENT)
Match location:
Position 4153 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTACACAACCCTCAGCAA
Hairpin Sequence:
5'-CCGG-GCAGTACACAACCCTCAGCAA-CTCGAG-TTGCTGAGGGTTGTGTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTACACAACCCTCAGCAACTCGAGTTGCTGAGGGTTGTGTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTACACAACCCTCAGCAACTCGAGTTGCTGAGGGTTGTGTACTGC-3'

Other clones with same target sequence:

(none)