Construct: shRNA TRCN0000081672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009628.1-1873s1c1
DNA Barcode:
GATAATTTAGAGGAGCCTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adnp (11538)
Gene Description:
activity-dependent neuroprotective protein
Transcript:
RefSeq NM_009628.1 (NON-CURRENT)
Match location:
Position 1873 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAATTTAGAGGAGCCTGTA
Hairpin Sequence:
5'-CCGG-GATAATTTAGAGGAGCCTGTA-CTCGAG-TACAGGCTCCTCTAAATTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAATTTAGAGGAGCCTGTACTCGAGTACAGGCTCCTCTAAATTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAATTTAGAGGAGCCTGTACTCGAGTACAGGCTCCTCTAAATTATC-3'

Other clones with same target sequence:

(none)