Construct: shRNA TRCN0000081795

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020331.1-1893s1c1
DNA Barcode:
GAGATGCTGTTCAACACGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gtf2ird1 (57080)
Gene Description:
general transcription factor II I repeat domain-containing 1
Transcript:
RefSeq NM_020331.1 (NON-CURRENT)
Match location:
Position 1893 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081795)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATGCTGTTCAACACGAAA
Hairpin Sequence:
5'-CCGG-GAGATGCTGTTCAACACGAAA-CTCGAG-TTTCGTGTTGAACAGCATCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATGCTGTTCAACACGAAACTCGAGTTTCGTGTTGAACAGCATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATGCTGTTCAACACGAAACTCGAGTTTCGTGTTGAACAGCATCTC-3'

Other clones with same target sequence:

(none)