Construct: shRNA TRCN0000081805

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009284.1-1885s1c1
DNA Barcode:
CGGCTGATCATTGGCTTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stat6 (20852)
Gene Description:
signal transducer and activator of transcription 6
Transcript:
RefSeq NM_009284.1 (NON-CURRENT)
Match location:
Position 1885 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081805)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTGATCATTGGCTTTATT
Hairpin Sequence:
5'-CCGG-CGGCTGATCATTGGCTTTATT-CTCGAG-AATAAAGCCAATGATCAGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTGATCATTGGCTTTATTCTCGAGAATAAAGCCAATGATCAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTGATCATTGGCTTTATTCTCGAGAATAAAGCCAATGATCAGCCG-3'

Other clones with same target sequence:

(none)