Construct: shRNA TRCN0000081821

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025847.1-967s1c1
DNA Barcode:
CGGGATGTCCATTTGTCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pbrm1 (66923)
Gene Description:
polybromo 1
Transcript:
RefSeq NM_025847.1 (NON-CURRENT)
Match location:
Position 967 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081821)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGATGTCCATTTGTCCAAA
Hairpin Sequence:
5'-CCGG-CGGGATGTCCATTTGTCCAAA-CTCGAG-TTTGGACAAATGGACATCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGATGTCCATTTGTCCAAACTCGAGTTTGGACAAATGGACATCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGATGTCCATTTGTCCAAACTCGAGTTTGGACAAATGGACATCCCG-3'

Other clones with same target sequence:

  1. TRCN0000302345