Construct: shRNA TRCN0000081898

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172918.3-2388s1c1
DNA Barcode:
CCTGAGTTGTTATGCAGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp317 (244713)
Gene Description:
zinc finger protein 317
Transcript:
RefSeq NM_172918.3 (NON-CURRENT)
Match location:
Position 2388 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081898)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGTTGTTATGCAGCAAT
Hairpin Sequence:
5'-CCGG-CCTGAGTTGTTATGCAGCAAT-CTCGAG-ATTGCTGCATAACAACTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGTTGTTATGCAGCAATCTCGAGATTGCTGCATAACAACTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGTTGTTATGCAGCAATCTCGAGATTGCTGCATAACAACTCAGG-3'

Other clones with same target sequence:

(none)