Construct: shRNA TRCN0000081920

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173384.1-2108s1c1
DNA Barcode:
CCTTTCAGAGACTATCCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sox30 (214105)
Gene Description:
SRY (sex determining region Y)-box 30
Transcript:
RefSeq NM_173384.1 (NON-CURRENT)
Match location:
Position 2108 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081920)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCAGAGACTATCCAGAT
Hairpin Sequence:
5'-CCGG-CCTTTCAGAGACTATCCAGAT-CTCGAG-ATCTGGATAGTCTCTGAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCAGAGACTATCCAGATCTCGAGATCTGGATAGTCTCTGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCAGAGACTATCCAGATCTCGAGATCTGGATAGTCTCTGAAAGG-3'

Other clones with same target sequence:

(none)