Construct: shRNA TRCN0000081946

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020618.3-1149s1c1
DNA Barcode:
GCATGGAGAAAGGAGAACCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smarce1 (57376)
Gene Description:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Transcript:
RefSeq NM_020618.3 (NON-CURRENT)
Match location:
Position 1149 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081946)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGGAGAAAGGAGAACCTT
Hairpin Sequence:
5'-CCGG-GCATGGAGAAAGGAGAACCTT-CTCGAG-AAGGTTCTCCTTTCTCCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGGAGAAAGGAGAACCTTCTCGAGAAGGTTCTCCTTTCTCCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGGAGAAAGGAGAACCTTCTCGAGAAGGTTCTCCTTTCTCCATGC-3'

Other clones with same target sequence:

(none)