Construct: shRNA TRCN0000081970
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_010658.2-1090s1c1
- DNA Barcode:
- CGCCCAGTCGTGCAGGTATAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mafb (16658)
- Gene Description:
- v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian)
- Transcript:
- RefSeq NM_010658.2 (NON-CURRENT)
- Match location:
- Position 1090 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000081970)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGCCCAGTCGTGCAGGTATAA
- Hairpin Sequence:
-
5'-CCGG-CGCCCAGTCGTGCAGGTATAA-CTCGAG-TTATACCTGCACGACTGGGCG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGCCCAGTCGTGCAGGTATAACTCGAGTTATACCTGCACGACTGGGCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGCCCAGTCGTGCAGGTATAACTCGAGTTATACCTGCACGACTGGGCG-3'
Other clones with same target sequence:
-
TRCN0000311788
