Construct: shRNA TRCN0000081972

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010658.2-548s1c1
DNA Barcode:
CCCAGCTTCAGTCCGACTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mafb (16658)
Gene Description:
v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian)
Transcript:
RefSeq NM_010658.2 (NON-CURRENT)
Match location:
Position 548 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000081972)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGCTTCAGTCCGACTGAA
Hairpin Sequence:
5'-CCGG-CCCAGCTTCAGTCCGACTGAA-CTCGAG-TTCAGTCGGACTGAAGCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGCTTCAGTCCGACTGAACTCGAGTTCAGTCGGACTGAAGCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGCTTCAGTCCGACTGAACTCGAGTTCAGTCGGACTGAAGCTGGG-3'

Other clones with same target sequence:

  1. TRCN0000349400