Construct: shRNA TRCN0000082032

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138596.1-162s1c1
DNA Barcode:
CTGAGCCAGAAGCTAAACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Med10 (28077)
Gene Description:
mediator complex subunit 10
Transcript:
RefSeq NM_138596.1 (NON-CURRENT)
Match location:
Position 162 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082032)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGCCAGAAGCTAAACTTT
Hairpin Sequence:
5'-CCGG-CTGAGCCAGAAGCTAAACTTT-CTCGAG-AAAGTTTAGCTTCTGGCTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGCCAGAAGCTAAACTTTCTCGAGAAAGTTTAGCTTCTGGCTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGCCAGAAGCTAAACTTTCTCGAGAAAGTTTAGCTTCTGGCTCAG-3'

Other clones with same target sequence:

  1. TRCN0000302125