Construct: shRNA TRCN0000082168

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173780.2-1357s1c1
DNA Barcode:
CGGCTCTTCATATTCCACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klf8 (245671)
Gene Description:
Kruppel-like factor 8
Transcript:
RefSeq NM_173780.2 (NON-CURRENT)
Match location:
Position 1357 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082168)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTCTTCATATTCCACTTT
Hairpin Sequence:
5'-CCGG-CGGCTCTTCATATTCCACTTT-CTCGAG-AAAGTGGAATATGAAGAGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTCTTCATATTCCACTTTCTCGAGAAAGTGGAATATGAAGAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTCTTCATATTCCACTTTCTCGAGAAAGTGGAATATGAAGAGCCG-3'

Other clones with same target sequence:

(none)